| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for specific detection of nucleic acids detecting the target nucleic acid from a mixture of various nucleic acid mixture on a solid phase. SOLUTION: The method comprises following steps. (A) providing a surface having immobilized nucleic acids or nucleic-acid analogues, which are suitable for forming non-covalent (base-pair) bonds with the target nucleic acids, (B) non-stringent hybridization of the target nucleic acids to be detected onto the immobilized nucleic acids from a solution of the analyte nucleic acid, (C) labeling of the nucleic acids of the analysis mixture with labeling elements, (D) optionally repeated treatment of the surface with a washing liquid in order to remove weakly bound nucleic acids, and (E) detection of the nucleic-acid pairs remaining on the surface with the aid of the labeling unit bonded to them by means of an optical, optical-spectroscopic, electrical, mechanical or magnetic detection method, wherein steps (B) and (C) can be carried out in optional order. COPYRIGHT: (C)2005,JPO&NCIPI
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