Methods of creating mutations in genomic exons by inserting introns into the genomic exons via homologous recombination. Also, methods are provided for introducing modifications into genomic exons by inserting introns into the genomic exons via homologous recombination such that a mature mRNA transcript produced from a genomic region of the genome comprising the genomic exon does not contain the modification are provided. The methods provide for a rapid method for introducing mutations and/or modifications of any type into a mammalian cell genome.
申请公布号
WO2005001087(A2)
申请公布日期
2005.01.06
申请号
WO2004US18964
申请日期
2004.06.10
申请人
REGENERON PHARMACEUTICALS, INC.;ECONOMIDES, ARIS, N.;VALENZUELA, DAVID, M.;DAVIS, SAMUEL;YANCOPOULOS, GEORGE
发明人
ECONOMIDES, ARIS, N.;VALENZUELA, DAVID, M.;DAVIS, SAMUEL;YANCOPOULOS, GEORGE