METHOD FOR PREPARING DHA AND EPA FROM MARINE DINOFLAGELLATE, LINGULODINIUM POLYEDRUM BY SIMPLE PROCESSING STEPS WITH HIGH PURITY AND HIGH YIELD

摘要

PURPOSE: Provided is a method for preparing DHA(docosahexaenoic acid) and EPA(eicosapentaenoic acid) useful for food additives, which uses Lingulodinium polyedrum as a starting material to produce DHA and EPA with high purity and high yield. CONSTITUTION: The method for preparing DHA and EPA uses a dry product containing DHA and EPA obtained by freeze drying Lingulodinium polyedrum, which is dinoflagellate. The method comprises the steps of: extracting the dry product with a mixed solvent; evaporating the organic solvent layer under reduced pressure and purifying the resultant product by chromatography, or evaporating the solvent under reduced pressure and extracting the resultant residue with lower alcohol/C6-8 alkane solvent, so that fractions of lower alcohol layer can be obtained; evaporating the fractions to remove the solvent; extracting the resultant residue with ethyl acetate/water to obtain the ethyl acetate layer; mixing the ethyl acetate layer with the C6-8 alkane layer; evaporating the resultant mixture under reduced pressure and purifying the residue by chromatography.