Bioassay for myostatin

摘要

The present invention relates to a bioassay for determining the amount of active myostatin present in an animal or part thereof comprising of steps (a) amplifying the myostatin promoter, or a functional or variant thereof; (b) cloning the promoter into a reporter vector or construct; (c) transfecting the vector into a myogenic precursor cell line (myoblasts) and selecting transfected myoblasts; (d) incubating transfected myoblasts with myostatin, serum or muscle extracts taken from animal of interest; (e) washing the myoblasts and preparing protein extracts therefrom; (h) performing a reporter gene assay on the protein extracts.