发明名称 |
AN IMPROVED GENETIC SCREEN FOR INTERACTION INTERFACE MAPPING |
摘要 |
<p>The present invention provides improved reverse hybrid assay methods for identifying amino acid residues within a protein that are required for its interaction or physical association with another protein, wherein disruption of an interaction between a protein of interest and its binding partner protein is assayed for a library of mutations of said protein of interest, and maintenance of an interaction between the protein of interest and another binding partner is assayed simultaneously in a single step, thereby reducing the incidence of uninformative mutations in the protein of interest that are detected.</p> |
申请公布号 |
WO2004106543(A1) |
申请公布日期 |
2004.12.09 |
申请号 |
WO2004AU00723 |
申请日期 |
2004.05.31 |
申请人 |
PHYLOGICA LIMITED;WATT, PAUL MICHAEL;HOPKINS, RICHARD;BOGOYEVITCH, MARIE |
发明人 |
WATT, PAUL MICHAEL;HOPKINS, RICHARD;BOGOYEVITCH, MARIE |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|