| 摘要 |
<p>A serological assay of an albumin-containing specimen for a predetermined antibody, which assay comprises: (i) contacting the specimen with an assay reactant which recognises the antibody in the presence of a dilution buffer comprising: (a) 5',5'-dibromo-O-cresolsulphonephthalein or a water-soluble salt thereof, (b) at least one blocking agent which is capable of suppressing non-specific binding in the assay and in whose presence a discernible change in the light absorption of component (a) can take place when the dilution buffer is contacted with albumin; and (c) a buffer which is capable of maintaining a pH at which the assay is able to be effected and the light absorption of component (a) is able to be changed; (ii) determining whether the light absorption of component (a) of the dilution buffer has changed; and (iii) determining the presence of the antibody in the specimen.</p> |
