发明名称 |
METHOD AND APPARATUS FOR RECOGNIZING MOLECULAR COMPOUNDS |
摘要 |
<p>A probe-target reaction is made more recognizable by the provision of a mass-enhancing and/or evanescent-field-perturbing amplifier element which reacts uniquely with and binds to the probe-target pair to provide increased mass. Where the probe-target pair is hybridized dsDNA, a suitable mass enhancing amplifier is anti-double stranded DNA mouse IgM. In examples with sufficient sequence pairs in the probe-target combination, a sequence-specific minor-groove-binding polyamide can be used that carries biotin which can be amplified by streptavidin in a suitable carrier. In a preferred embodiment, a plurality of probes are immobilized at the sites of a microarray, each probe being specific to a different target. Optics utilizing total internal reflection are described for observing perturbation of the evanescent field.</p> |
申请公布号 |
WO2004106357(A1) |
申请公布日期 |
2004.12.09 |
申请号 |
WO2003US17137 |
申请日期 |
2003.05.28 |
申请人 |
MAVEN TECHNOLOGIES, LLC;RASSMAN, WILLIAM;RALIN, DAVID;ZHOU, FEIMENG;HAN, SHUBO |
发明人 |
RASSMAN, WILLIAM;RALIN, DAVID;ZHOU, FEIMENG;HAN, SHUBO |
分类号 |
G01N21/55;C07H21/04;G01N21/21;G01N21/75;G01N33/543;(IPC1-7):C07H21/04;C12M3/00;C12Q1/68;G01N21/00;G01N21/17;G01N33/00;G01N33/53;G01N33/557;G01J4/00 |
主分类号 |
G01N21/55 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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