HIGH-THROUGHPUT METHODS OF SCREENING DNA FOR DELETIONS AND OTHER MUTATIONS

摘要

The subject invention relates to high-throughput methods of screening DNA fo r mutations. These methods offer various unexpected advantages over current methods. In a preferred embodiment, the subject invention includes pooling D NA samples from many plants that were subjected to mutagenesis. Methods of the subject invention include highly sensitive means for detecting individual mutants, preferably deletions, in large pools or collections of DNA samples.