ISOLATED STRAINS OF AKABANE VIRUS, CHUZAN VIRUS AND AINO VIRUS, AND THE METHOD FOR PREPARING THE VIRAL VACCINE BY MIXING THE SAME

摘要

A viral vaccine containing isolated virus strains is provided to improve use safety and immunogenicity by using inactivated viruses, and increase production yield of cows, so that the vaccine is useful for prevention of abortion or stillbirth in cows caused by viruses via mediation of Culicoides spp. A viral vaccine for prevention of abortion or stillbirth in cows contains cultured medium of inactivated virus strains of akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P), and is produced by: inactivating akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P) by adding 0.1M BEI(binary ethylene imine) prepared by solubilizing 2-BEA(2-bromoethylamine) in 0.2N NaOH, and treating the solution in a constant temperature water bath of 37 deg. C for 1 hour to them; mixing the 3 kinds of viruses in a weight ratio of 1:1:1; and adding 40-60 wt.% of IMS 1314 as an immunomodulator into the viral mixture.