| 摘要 |
To detect intracellular negatively supercoiled DNA conveniently and efficiently. A method for detecting negatively supercoiled DNA in cells, characterized by including the steps of incorporating biotinylated psoralen into cells, irradiating the cells with long-wavelength UV rays, causing the cells to react with adivin which has been labeled with a color-developing substance, a fluorescent substance, or a chemiluminescent substance, and measuring developed color, emitted fluorescence, or emitted chemiluminescence of the cells.
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