| 摘要 |
A method is described for the specific detection of nucleic acids on a solid phase. The method involves the steps of: A) providing a surface having immobilized nucleic acids or nucleic-acid analogues, which are suitable for forming non-covalent (base-pair) bonds with the target nucleic acids, B) non-stringent hybridization of the target nucleic acids to be detect ed onto the immobilized nucleic acids from a solution of the analyte nucleic acid, C) labeling of the nucleic acids of the analysis mixture with labeling elements, D) optionally repeated treatment of the surface with a washing liquid i n order to remove weakly bound nucleic acids, and E) detection of the nucleic-acid pairs remaining on the surface with th e aid of the labeling unit bonded to them, by means of optical, optical- spectroscopic, electrical, mechanical or magnetic detection methods; wherein steps B) and C) can be carried out in any order.
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