Sensitive diagnostic testing methodology using multiplex real time PCR with one dye (MOD) and its use as in severe acute respiratory syndrome (SARS)

摘要

This invention provides a new and more sensitive methodology for multiplex real time PCR testing of any genetic materials. The method is best illustrated here with the detection and diagnosis of Severe Acute Respiratory Syndrome (SARS) but can be applicable to any other usage where more sensitivity is required. It can be combined with the usual multiplex real time PCR test for further confirmation and multiple parameter assessment of SARS or other diseases. A kit form is provided for the detection of SARS by this methodology, but equally applicable to other diseases or testing conditions.