发明名称 |
CLEAVAGE OF FUSION PROTEINS USING GRANZYME B PROTEASE |
摘要 |
A method for the preparation of a polypeptide of interest in authentic form by enzymatic cleavage of fusion proteins using Granzyme B protease (EC 3.4.21.79). There is also provided fusion proteins comprising a polypeptide of interest and a fusion partner, wherein the junction region between the polypeptide of interest and the fusion partner comprises a Granzyme B protea se cleavage site adjacent to the polypeptide of interest, and a human Granzyme B protease variant wherein the Cystein residue no. 228 (chymotrypsinogen numbering) is mutated to Phenylalanine.
|
申请公布号 |
CA2523101(A1) |
申请公布日期 |
2004.11.04 |
申请号 |
CA20042523101 |
申请日期 |
2004.04.23 |
申请人 |
BOREAN PHARMA A/S |
发明人 |
LORENTSEN, RIKKE HOEGH;FYNBO, CHARLOTTE HARKJAER |
分类号 |
C12N9/64;(IPC1-7):C07K19/00 |
主分类号 |
C12N9/64 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|