| 摘要 |
Disclosed are compounds of the formula (I): wherein R<3>, R<4>, R<5>, R<9>, and R<10> are selected from the group consisting of H and groups or atoms other than H, and R<6> and R<8> are halo or hydrogen; X<1>, X<2>, and X<3> are independently O or S; provided that R<9> and R<10> are not simultaneously H, when all of X<1> , X<2>, and X<3> are O; and of the formula (II) wherein R<11>-R<14> are selected from the group consisting of H and groups or atoms other than H; X<4>- X<9> are independently O or S; n and m are 0 or 1 but m and n cannot be 0 simultaneously; R<15>- R<24> can be H or any substituent so long as the compound of formula II upon hydrolysis provides a fluorescent compound. These compounds are useful as substrates with high specificity for organophosphatase particularly human paraoxonase and bacterial organophosphorus hydrolase. Also disclosed is a method for detecting and/or measuring the paraoxonase activity in a fluid comprising contacting the fluid with a fluorescent substrate and measuring the fluorescence of the fluorescent product formed. |
