cAMP RECEPTOR PROTEIN AND ADENYLATE CYCLASE OF VIBRIO VULNIFICUS AS GLOBAL VIRULENCE REGULATORS TO REGULATE VIRULENCE EXPRESSION

摘要

PURPOSE: cAMP receptor protein and adenylate cyclase of Vibrio vulnificus as global virulence regulators to regulate virulence expression are provided. Therefore, the host cell is protected from infection of Vibrio vulnificus by deleting genes encoding the cAMP receptor protein and adenylate cyclase. CONSTITUTION: The crp gene encoding the cAMP receptor protein(CRP) of Vibrio vulnificus to regulate virulence expression has the nucleotide sequence of SEQ ID NO:1, wherein the cAMP receptor protein(CRP) of Vibrio vulnificus has the amino acid sequence of SEQ ID NO:2. The cya gene encoding the adenylate cyclase of Vibrio vulnificus which binds to the CRP to activate the CRP has the nucleotide sequence of SEQ ID NO:3, wherein the adenylate cyclase of Vibrio vulnificus has the amino acid sequence of SEQ ID NO:4.