发明名称 |
AFFINITY PURIFICATION SYSTEM USING TROPONIN MOLECULES AS AFFINITY LIGANDS |
摘要 |
This invention pertains to the use of a novel set of tags for the immobilization and/or purification of proteins or other biological or organic molecules. The invention provides troponin C, troponin C binding peptide, and troponin I, or active fragments or analogues thereof as convenient tags and affinity ligands for immobilizing, attaching, or purifying proteins or other molecules. Methods for producing troponin-tagged molecules, such as recombinant fusion proteins, are described. Methods for preparing a troponin affinity matrix that is capable of specifically binding its cognate ligand in the presence of calcium, and methods for using such a matrix to purify troponin-tagged molecules are also described. |
申请公布号 |
WO2004092214(A2) |
申请公布日期 |
2004.10.28 |
申请号 |
WO2004US10887 |
申请日期 |
2004.04.07 |
申请人 |
ARISTEX;MOECKLI, RANDOLPH, A.;CHADWICK, CHRISTOPHER, C. |
发明人 |
MOECKLI, RANDOLPH, A.;CHADWICK, CHRISTOPHER, C. |
分类号 |
C07K14/47;C12N15/62 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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