发明名称 |
METHOD FOR PREPARATION OF SITE-SPECIFIC PROTEIN CONJUGATES |
摘要 |
<p>The invention is directed to a single-step method for rapidly and efficientl y preparing protein-polymer conjugates, including an insulin-polymer conjugate . According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB 1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradabl e polymer.</p> |
申请公布号 |
CA2521381(A1) |
申请公布日期 |
2004.10.28 |
申请号 |
CA20042521381 |
申请日期 |
2004.04.08 |
申请人 |
PR PHARMACEUTICALS INC. |
发明人 |
HINDS, KENNETH;SCHMIDT, PAUL;LEWIS, DANNY;CAMPBELL, KATHLEEN M. |
分类号 |
C07K17/08;A61K;A61K31/00;A61K38/28;A61K47/48;C07K1/18;C07K14/62 |
主分类号 |
C07K17/08 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|