AAV vector packaging plasmid for producing wtaav particles or pseudotyped aav particles without helper viruses, by means of a single transfection

摘要

The present invention relates to AAV vector packaging plasmids for the helper virus-free preparation of (pseudotyped) AAV particles by means of single transfection. The AAV vector packaging plasmids for the (pseudotyped) AAV particles comprise the following DNA sequences: (a) a rep gene of AAV, (b) a cap gene of AAV, (c) AAV expression vector DNA sequences, and (d) all further helper virus DNA sequences necessary for forming AAV particles. The AAV vector packaging plasmids for the preparation of wtAAV particles are characterized in that they (a) comprise the complete AAV genome and (b) all of the helper virus DNA sequences necessary for forming AAV particles. The invention also relates to the use of these AAV vector packaging plasmids for preparing wtAAV particles or pseudotyped AAV particles in particular for gene therapy or tumor therapy.