METHOD FOR SEPARATION OF ANTITHROMBIN

摘要

A method for separation of native antithrombin and pre-latent antithrombin present in a sample from each other is provided. The method comprises performing, in the order given, the following steps: a) application of a sample containing native antithrombin and pre-latent antithrombin to an affinity material with affinity for antithrombin, wherein said application is performed under conditions such that binding of native antithrombin and pre-latent antithrombin to the affinity material is achieved; b) application of a solution of an elution salt S1 to said affinity material at a concentration C1 of elution salt, for elution of pre-latent antithrombin, said concentration C1 is in the range of 30-95 % of the concentration needed for elution of native antithrombin with the elution salt S1. In the method, the concentration C1 is such that pre-latent antithrombin is eluted and native antithrombin is retained.