PREPARATION OF A METAL CHELATING SEPARATION MEDIUM
摘要
<p>The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula below R-O-CO-(CH2)n-CHNH2-CO-OH wherein R is a selectively cleavable group; and n is an integer of 1-4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.</p>
申请公布号
WO2004087284(A1)
申请公布日期
2004.10.14
申请号
WO2004SE00502
申请日期
2004.04.01
申请人
AMERSHAM BIOSCIENCES AB;ANDERSSON, LARS, C.;GEBRU, TESFAI;AHMAD, TANIA;CLACHAN, JOHN;AXEN, ANDREAS
发明人
ANDERSSON, LARS, C.;GEBRU, TESFAI;AHMAD, TANIA;CLACHAN, JOHN;AXEN, ANDREAS