COMPOSITIONS, METHODS AND SYSTEMS FOR THE PRODUCTION OF ENEDIYNES

摘要

Five protein families cooperate to form the warhead structure that characterizes enediyne compounds, both chromoprotein enediynes and non-chromoprotein enediynes. The protein families include a polyketide synthase and thioestera se protein which form a polyketide synthase catalytic complex involved in warhead formation in enediynes. Genes encoding a member of each of the five protein families are found in all enediyne biosynthetic loci. The genes and proteins may be used in geneti c engineering applications to design new enediyne compounds and in methods to identify new enediyne biosynthetic loci.