发明名称 |
Recombinant expression of hhbv reverse transcriptase (rt) |
摘要 |
The present invention relates to a method of producing a functional cell-free hepatitis B virus (HBV) reverse transcriptase (RT) comprising the steps of expressing HBV-RT in E. coli cells employing a suitable expression plasmid, or in a cell-free transcription-translation system and if expression was carried out in E. coli cells, lysing said E. coli cells and purifying HBV-RT from E. coli lysate or from said transcription-translation system. The invention further relates to a method of screening for an inhibitor of HBV-RT activity comprising the steps of contacting the cell-free HBV-RT produced according to methods of the present invention and with a potential inhibitor and assaying whether said potential inhibitor inhibits HBV-RT activity. Furthermore, the invention provides for a method of producing a pharmaceutical composition comprising the step of formulating the inhibitor identified by the screening method of the invention into a pharmaceutical composition.
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申请公布号 |
US2004197889(A1) |
申请公布日期 |
2004.10.07 |
申请号 |
US20040476056 |
申请日期 |
2004.04.21 |
申请人 |
BUCHNER JOHANNES;MUSCHLER PAUL;HASLBECK MARTIN |
发明人 |
BUCHNER JOHANNES;MUSCHLER PAUL;HASLBECK MARTIN |
分类号 |
C12N15/09;A61K36/00;A61K36/47;A61K45/00;A61P1/16;A61P31/20;A61P43/00;C12N9/12;C12N15/10;C12N15/62;C12N15/70;C12P19/34;C12Q1/18;C12Q1/48;G01N33/68;(IPC1-7):C12N9/22;C07H21/04;C12N15/86 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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