MICROSPHERE COMPOSITION OF SUSPENSION ARRAY FOR ANALYZING HUMAN PAPILLOMAVIRUS(HPV) GENOTYPE AND ANALYSIS METHOD OF HPV GENOTYPE BY REACTION OF HPV PCR PRODUCTS AND MICROSPHERE BEADS

摘要

PURPOSE: Provided are a microsphere composition of suspension array for analyzing Human Papillomavirus (HPV) genotype and an analysis method of the genotype of HPV by reacting HPV PCR products with microsphere beads. Therefore, the microsphere composition is useful for diagnosis of cancer, and prevention and treatment of HPV infection. CONSTITUTION: A plurality of oligonucleotide probe compositions for analyzing Human Papillomavirus (HPV) genotype comprise the nucleotide sequences set forth in SEQ ID NO:1 to SEQ ID NO:32, wherein the oligonucleotide probes are immobilized into microspheres. The method for analyzing Human Papillomavirus (HPV) genotype comprises the steps of: (a) carrying out OTN(one tube nested) asymmetric PCR with a biotin-attached primer; (b) binding the biotin-attached OTN asymmetric PCR product with the oligonucleotide probe by simultaneously binding streptavidin-Cy3 or streptavidin-phycoerythrin with the PCR product in one tube; and (c) verifying the binding of the OTN asymmetric PCR product with the oligonucleotide probe using Luminex 100 LabMAP.