| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for increasing transformation efficiency of hematopoietic cell and other cells. <P>SOLUTION: The method to increase the efficiency of transformation of hematopoietic cell and other cells by retroviruses comprises infecting the cells in the presence of fibronectin or fibronectin fragments. The fibronectin and fibronectin fragments significantly enhance retroviral-mediated gene transfer into the cells, particularly hematopoietic cells including committed progenitors and primitive hematopoietic stem cells. The improved methods for somatic gene therapy capitalizing on enhanced gene transfer, hematopoietic cellular populations, and novel constructs for enhancing retroviral-mediated DNA transfer into cells are provided and their uses are also provided. <P>COPYRIGHT: (C)2004,JPO&NCIPI |
