METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYING IBPA AND/OR IBPB GENE ENCODING INCLUSION BODY-ASSOCIATED PROTEINS

摘要

PURPOSE: A method for producing target proteins by deleting or amplifying ibpA and/or ibpB gene encoding inclusion body-associated proteins is provided, thereby inducing secretion of water-soluble protein having its activity, and improving productivity of target proteins which are produced in a type of inclusion body within cytoplasm of a bacterium having the amplified ibpA and/or ibpB gene. CONSTITUTION: The method for producing target proteins by deleting the ibpA and/or ibpB gene encoding inclusion body-associated proteins comprises culturing the ibpA and/or ibpB gene deleted bacterium and a gene encoding the target gene, wherein the ibpA and/or ibpB gene deleted bacterium is Escherichia coli WIB101; the bacterium further contains secretion signal sequence; and the target protein is leptin or alkaline phosphatase. The method for producing target proteins by amplifying the ibpA and/or ibpB gene encoding inclusion body-associated proteins comprises culturing the ibpA and/or ibpB gene amplified bacterium and a gene encoding the target gene, wherein the ibpA and/or ibpB gene deleted bacterium is produced by using plasmid pACTacIbpAB, pACIbpAB, pACTacIbpA or pACTacIbpB which can amplify the ibpA and/or ibpB gene; the ibpA and/or ibpB gene deleted bacterium is Escherichia coli; the target proteins are produced within a water-insoluble inclusion body; the target proteins are accumulated in cytoplasm of the bacterium; and the target protein is insulin-like growth factor-I, interleukin 12 beta chain, interferon-gamma or green fluorescent protein(GFP).