A RECOMBINANT CONSTRUCTED BY A VIRUS VECTOR AND A HUMAN TUMOR SUPPRESSOR GENE AND ITS USE

摘要

The invention relates to a recombinant virus, which is engineered to contain a human tumor suppressor gene, and its application. The recombinant constructed by DNA cloning technology is combined with virus vector and human tumor suppressor gene expression cassette, which can amplify and propagate in specific genetic-engineered cell line, and also can express tumor suppressor protein in eukaryote cells. The recombinant adenovirus-p53 DNA is constructed with adenoviral vector and human p53 gene by homologous recombination in E. Coli. The human p53 expression cassette, comprising promoter-p53 cDNA-poly (A), is a specific DNA sequence. The recombinant adenovirus-p53 can be prepared as clinical gene therapy products, which apply in treating and preventing malignant tumors.