DNA AND AMINO ACID SEQUENCES OF A TYROSINE-INDUCIBLE TYROSINE AMMONIA LYASE ENZYME FROM THE YEAST TRICHOSPORON CUTANEUM
摘要
A novel tyrosine-inducible tyrosine ammonia lyase enzyme was isolated from the yeast Trichosporon cutaneum. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of para-hydroxycinnamic acid directly from tyrosine. The gene encoding this enzyme was sequenced using 3 and 5 RACE cloning of the TAL cDNA and the gene was expressed in the yeast Saccharomyces cerevisiae and in the bacterium Escherichia coli.