COMPOSITION FOR DIAGNOSIS OF POLYCYSTIC OVARY SYNDROME COMPRISING PRIMERS DETECTING SINGLE NUCLEOTIDE POLYMORPHISM AT 677TH NUCLEOTIDE OF 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE GENE

摘要

PURPOSE: A composition for diagnosis of polycystic ovary syndrome comprising primers detecting single nucleotide polymorphism at 677th nucleotide of 5,10-methylenetetrahydrofolate reductase gene is provided, thereby improving accuracy of diagnosis of polycystic ovary syndrome. CONSTITUTION: The composition for diagnosis of polycystic ovary syndrome comprises primers detecting single nucleotide polymorphism at 677th nucleotide of 5,10-methylenetetrahydrofolate reductase(MTHFR) gene, wherein the primers contain a sense primer having the nucleotide sequence set forth in SEQ ID NO:1 or SEQ ID NO:3 and an antisense primer having the nucleotide sequence set forth in SEQ ID NO:2 or SEQ ID NO:4; the primers further contain a sequence primer for pyrosequencing having the nucleotide sequence set forth in SEQ ID NO:5; the single nucleotide polymorphism at 677th nucleotide of MTHFR gene is selected from cytosine/cytosine(C/C), cytosine/thymine(C/T) and thymine/thymine(T/T).