| 摘要 |
PURPOSE:To obtain the subject new DNA and recombinant DNA capable of coding an enzyme liberating trehalose from a nonreducing glucide having a terminal trehalose structure and a specific glucose polymerization degree and mass-producing the trehalose useful for addition, etc., to foods. CONSTITUTION:This new DNA is capable of coding an enzyme has functions to liberate trehalose from a nonreducing glucide having the trehalose structure at the terminal and >=3 glucose polymerization degree, has about 57000-68000Da [measured by the sodium dodecyl sulfate (SDS)-polyacrylamide gel e1 ectrophoresis] and about 3.3-4.6 isoelectric point (measured by the isoelectric focusing) and is capable of coding an enzyme comprising an amino acid sequence expressed by formula I or II or an amino acid sequence homologous therewith. Furthermore, this recombinant DNA contains the new DNA. The DNA is obtained by extracting a chromosomic DNA from a microorganism of the genus Arthrobacter, Brevibacterium or Micrococcus and treating the resultant chromosomic DNA with a restriction enzyme and joining the treated DNA to a vector. |
