COMPOSITION FOR ANTI-ANGIOGENESIS AND INHIBITING CANCER GROWTH COMPRISING KRINGLE DOMAIN PROTEIN TK1-2 AND USE THEREOF

摘要

PURPOSE: A composition for anti-angiogenesis and inhibiting cancer growth comprising kringle domain protein TK1-2 and use thereof are provided, thereby inhibiting growth of endothelial cell, inhibiting angiogenesis and inhibiting growth of cancer, so that it can be useful for prevention of disease caused by angiogenesis and cancer. CONSTITUTION: The composition for anti-angiogenesis and inhibiting cancer growth comprises kringle domain protein TK1-2 derived from human tissue type plasminogen activator(t-PA) and having the amino acid sequence set forth in SEQ ID NO: 1. A recombinant E. coli expression plasmid pET-15b/TK1-2 produces the kringle domain protein TK1-2. The recombinant Pichia expression plasmids His-TK1-2/pPICZα-C and N-TK1-2/pPICZα-C produce the kringle domain protein TK1-2. A transformed E. coli produced by transforming E. coli with the plasmid pET-15b/TK1-2. A transformed Pichia sp. (KCTC 10415BP) produced by transforming Pichia with N-TK1-2/pPICZα-C produces the protein TK1-2.