发明名称 |
METHOD FOR PROTEIN ISOLATION IN ANOXIC CONDITIONS |
摘要 |
The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as &bgr;-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells. |
申请公布号 |
CA2514416(A1) |
申请公布日期 |
2004.08.12 |
申请号 |
CA20042514416 |
申请日期 |
2004.01.29 |
申请人 |
AKZO NOBEL NV |
发明人 |
EPPINK, MICHEL HENDRIKUS MARIA;WASSENAAR, ROELAND WILHELMUS;VAN DER MEIJDEN, PETER;VAN DEDEM, GIJSBERT WILLEM KAREL |
分类号 |
C07K1/113;C07K1/36;C07K14/62 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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