发明名称 |
AFFINITY TRAP REACTOR AND SINGLE-STEP PROCESS FOR PURIFYING ANGIOSTATIN-LIKE FRAGMENT FROM HUMAN PLASMA USING THE SAME |
摘要 |
It is intended to provide an affinity trap reactor allowing efficient progre ss of a reaction between an enzyme bonded to a support and a substrate without restriction in use depending on the types of the enzyme and substrate employed. An affinity trap reactor comprising a support having an enzyme and a molecule specifically reacting with the substrate of the enzyme bonded thereto; and a process for obtaining BL-angiostatin in a single step from plasminogen contained in a biological sample which comprises applying the biological sample containing plasminogen to an affinity trap reactor comprising a support having bacillolysin MA and lysine bonded thereto and reacting at 0 to 50 ~C in the presence of isopropyl alcohol under calcium io n- free conditions to thereby obtain BL-angiostatin.
|
申请公布号 |
CA2512894(A1) |
申请公布日期 |
2004.08.05 |
申请号 |
CA20032512894 |
申请日期 |
2003.01.17 |
申请人 |
TTC CO., LTD. |
发明人 |
HASUMI, KEIJI;SHIMIZU, KOSUKE |
分类号 |
C12N9/68;C12N11/10;(IPC1-7):C12N11/00;C12P21/06 |
主分类号 |
C12N9/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|