发明名称 |
Methods for producing modified nucleic acid molecules |
摘要 |
A method for inserting a cassette into a nucleic acid molecule to produce a modified nucleic acid molecule, e.g., a nucleic acid-cassette fusion, is described. The method involves the use of polymerase chain reaction, thereby permitting precise targeting of the desired site in the molecule, but does not require ligation. Also described is the use of this method for high throughput screening the resulting modified nucleic acid molecules.
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申请公布号 |
US6770462(B1) |
申请公布日期 |
2004.08.03 |
申请号 |
US20010009315 |
申请日期 |
2001.11.05 |
申请人 |
SMITHKLINE BEECHAM CORPORATION;SMITHKLINE BEECHAM P.L.C. |
发明人 |
CHALKER ALISON F.;HOLMES DAVID J.;LUNSFORD ROBERT DWAYNE;GE JAMES YIGONG |
分类号 |
C07K14/315;C12N15/10;(IPC1-7):C12P19/34 |
主分类号 |
C07K14/315 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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