| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To implement a biological electron microscope and an observation method capable of observing a biosample with high contrast, without significant damages, and of analyzing images with high precision, and performing high-throughput sample pretreatments. <P>SOLUTION: (1) Observation is performed by an accelerating voltage 1.2 to 4.2 times the sample transmission critical accelerating voltage of electron beams which is determined under prescribed conditions. (2) An E×B type energy filter of a compact and simple structure is provided between the sample and an electron beam detector, and an image is formed by electron beams existing within a specific energy region among electron beams transmitted through the sample. (3) Quantitative analysis is carried out by image processings on similarities among observed images of viruses, proteins, etc. in the sample and reference images of known viruses, proteins, etc. (4) A biosample pretreatment protocol is formed into a chip through the use of the MEMS technology, and the chip is mounted on a sample stage part of the electron microscope and will carry out introduction, pretreatment, and until the transfer of the sample to a sample holder. <P>COPYRIGHT: (C)2004,JPO&NCIPI</p> |
