发明名称 |
Method for gene diagnosis of bovine Hsp70 deficiency |
摘要 |
A method for gene diagnosis of bovine Hsp70 deficiency, which comprises (a) a step of obtaining a bovine nucleic acid sample, (b) a step of subjecting the nucleic acid sample obtained in step (a) to a gene amplification reaction to obtain a nucleic acid fragment in which a region including a mutation site likely to be present in bovine Hsp70 gene is amplified, and (c) a step of examining the presence of mutation on the nucleic acid fragment in step (b), the region including the mutation site being a region including 1997-11030 position of a base sequence shown in SEQ ID No. 1 of SEQUENCE LISTING in a base sequence of bovine Hsp70 gene. According to the invention, bovine Hsp70 deficiency and its carrier can be detected and diagnosed by a genetic engineering method easily and quickly, and a kit used for this purpose is provided.
|
申请公布号 |
US2004146878(A1) |
申请公布日期 |
2004.07.29 |
申请号 |
US20030609181 |
申请日期 |
2003.06.26 |
申请人 |
DIRECTOR OF LIVESTOCK IMPROVEMENT ASSOCIATION OF JAPAN, INC. |
发明人 |
SUGIMOTO MAYUMI;FURUOKA HIDEFUMI;SUGIMOTO YOSHIKAZU |
分类号 |
C12N15/09;A61B10/00;C12N15/10;C12N15/12;C12P19/34;C12Q1/68;G01N33/53;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|