Fluorescence detection method and fluorescence detection device

摘要

A silver thin film (with a thickness of 50 nm) and an SiO2 film (with a thickness of 10 nm) are deposited in this order on one side surface of a slide glass by ion beam sputtering deposition, so that a supporting substrate for sample solution is formed. A sample solution containing fluorescence molecules is placed on the SiO2 film of the supporting substrate and a cover glass is placed on the sample solution. When light is entered upon the interface between the slide glass and the SiO2 film at an incident angle of 59 to 60°, surface plasmon resonance is excited at the interface and thus an evanescent field is reinforced, so that fluorescence is generated efficiently from the fluorescence molecules contained in the sample solution.