| 摘要 |
Compounds that are readily dissociable conjugates of a multiplicity of biologically active molecules are formed by bonding to each biologically active molecule one or more oligonucleotide chains selected to comprise partially complementary sequences that form duplexes with other such oligonucleotides attached to other biologically active molecules. The claimed conjugates and methods for preparing the conjugates of the invention can be used to link two or more biologically active molecules, as well as conjugating a multiplicity of biologically active molecules. In alternative embodiments, conjugates comprising such covalently linked oligonucleotides linked by one or a multiplicity of "bridging" oligonucleotides are provided. Methods for making these different types of conjugates and for using such conjugates for research, immunoassay, medical and technical applications are also provided by the invention. The disclosed methods provide for the formation of intermolecular links which are hydrophilic, of relatively low toxicity and antigenicity, are as flexible and extensible as desired, and are stable yet dissociable. Both the components and the conjugates of the invention can be isolated and purified with relative ease compared with other intermediates and conjugates.
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