发明名称 |
RECOMBINANT CYSTEINE PROTEINASE ISOLATED FROM PARAGONIMUS WESTERMANI AND KIT FOR DIAGNOSIS OF PARAGONIMUS WESTERMANI USING THE SAME |
摘要 |
PURPOSE: Recombinant cysteine proteinase isolated from Paragonimus westermani and a kit for diagnosis of Paragonimus westermani using the same are provided, thereby reducing cross-reaction with other parasites, and improving sensitivity of diagnosis. CONSTITUTION: The recombinant cysteine proteinase isolated from Paragonimus westermani is encoded by a gene having the nucleotide sequence set forth in SEQ ID NO: 1. The method for preparing the recombinant cysteine proteinase isolated from Paragonimus westermani comprises the steps of: designing primer 1 (5'-gaattcgctcccgaacgtattgac-3') which has a cysteine protease conserving region and is digested with a restriction enzyme EcoRI, and primer 2 (5'-gtcgaccatatcaccttccttatc-3') which is digested with a restriction enzyme SalI; reacting cDNA of Paragonimus westermani with the primers 1 and 2 to isolate a DNA fragment associated with cysteine protease; sequencing the isolated DNA fragment; and preparing cysteine protease using the sequenced DNA fragment. The kit for diagnosis of Paragonimus westermani comprises the recombinant cysteine protease as an antigen.
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申请公布号 |
KR20040063281(A) |
申请公布日期 |
2004.07.14 |
申请号 |
KR20030000637 |
申请日期 |
2003.01.06 |
申请人 |
DM CO., LTD. |
发明人 |
JUN, BYEONG HUN;KIM, SEOK IL;LEE, GIL HWAN;PARK, HYEON |
分类号 |
C12N15/57;(IPC1-7):C12N15/57 |
主分类号 |
C12N15/57 |
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