发明名称 |
Method for isotope labeling of protein with enzyme |
摘要 |
The present invention provides a method for isotopically labeling a functional group possessed by an amino acid residue of a protein. The present invention also provides a protein whose functional group in an amino acid residue is isotopically labeled.A functional group in an amino acid residue of a protein is substituted with an isotope-labeling group derived from an isotope-labeling compound by making use of the action of an enzyme. In particular, the carboxyamide nitrogen atom in a glutamine residue of a protein is replaced with an isotopically labeled atom by acting a transglutaminase on the glutamine residue.
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申请公布号 |
US6762041(B2) |
申请公布日期 |
2004.07.13 |
申请号 |
US20010850031 |
申请日期 |
2001.05.08 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
SHIMBA NOBUHISA;SUZUKI EIICHIRO;YOKOYAMA KEIICHI |
分类号 |
C07K5/072;G01N33/534;(IPC1-7):C12P13/14;C12N9/10 |
主分类号 |
C07K5/072 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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