发明名称 |
Functional assay for g-protein-coupled receptors based on insect cells |
摘要 |
The invention provides insect cell-based systems for assaying G-protein-coupled receptor interactions. The insect cells may express a heterologous mammalian G-protein-coupled receptor, a heterologous G alpha protein and one or more insect effector proteins that couple the heterologous G alpha protein to an endogenous insect signalling pathway. In such systems, binding of a ligand to the heterologous G-protein coupled receptor mediates a detectable change in the insect cell. The protein components of the system may be expressed from coding sequences that are stably maintained by transformed insect cells.
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申请公布号 |
US2004132107(A1) |
申请公布日期 |
2004.07.08 |
申请号 |
US20030415621 |
申请日期 |
2003.11.07 |
申请人 |
GRIGLIATTI THOMAS ANTHONY;KIRK KNIGHT PETER JOHN;PFEIFER THOMAS ARTHUR |
发明人 |
GRIGLIATTI THOMAS ANTHONY;KIRK KNIGHT PETER JOHN;PFEIFER THOMAS ARTHUR |
分类号 |
C07K14/705;G01N33/50;G01N33/566;(IPC1-7):G01N33/53;G01N33/567;C12N5/06 |
主分类号 |
C07K14/705 |
代理机构 |
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地址 |
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