A cell isolating device and method is provided to concentrate or isolate cells with specific characteristics from a mixture of different cell types. One embodiment may comprise two subtypes of antibodies that are directly conjugated to biotin (Abb) and conjugated to a fluorescent molecule (Abf). The conjugated antibodies (Abb +Abf) bind to the target cells in a mixed cell suspension. The cell suspension is then passed over an immobilized avidin or streptavidin substrate on a glass microscope slide. The biotinylated target cells adhere to the avid in/streptavidin substrate, while the unbound cells are washed off and collected in a wicking member. Captured cells on the avid in/streptavidin substrate may then be visualized directly using a fluorescent microscope or detected and enumerated via an on-board fluorescent detection device.
申请公布号
WO2004027021(A3)
申请公布日期
2004.07.08
申请号
WO2003US21961
申请日期
2003.07.10
申请人
NEXELL OF CALIFORNIA, INC.;BERSTEIN, STEVE;ROSS, AMELIA, A., PH.D