MEDIUM COMPOSITION AND CULTURE METHOD FOR IMPROVING PREIMPLANTATION DEVELOPMENT OF CLONE EMBRYO

摘要

PURPOSE: A medium composition and culture method for improving preimplantation development of clone embryo are provided, thereby effectively inhibiting apoptosis of blastomere to improve preimplantation development of clone embryo, thereby improving the production yield of health clone embryo. CONSTITUTION: The medium composition for in vitro culturing the nucleus transferring somatic cell for preparing clone embryo contains 1-25 micromole of antibiotics such as beta-mercaptoethanol, and 1-10 microgram/milliliter of an oxidized nitrogen removing factor such as hemoglobin. The method for in vitro culturing the clone embryo in the medium composition comprises the steps of: (a) in vitro culturing the nucleus transferring somatic cell in the in vitro culturing medium composition containing antibiotics; (b) transplanting the nucleus of the nucleus transferring somatic cell to a nucleus receiving egg of which genetic materials are removed to prepare somatic cell clone embryo; and (c) in vitro culturing the somatic cell clone embryo in the in vitro culturing medium composition containing antibiotics and oxidized nitrogen removing factor.