Mutant E. coli strains, and their use for producing recombinant polypeptides

摘要

A process for producing predetermined recombinant polypeptides or proteins, comprising expressing the polypeptides or proteins in Escherichia coli (E. coli) strains whose gene coding RNase E comprises a mutation such that the enzyme produced upon expression of this mutated gene exhibits reduced activity for degrading the messenger RNA (m-RNA) encoding the polypeptides or proteins, compared to bulk mRNA, the mutation not significantly affecting growth of the E. coli strains, and wherein the mutation corresponds to the substitution or deletion of one up to all the nucleotides located in the region delimited by the nucleotide at position 2193 and the nucleotide at position 2975 of the DNA sequence coding the RNase E represented by SEQ ID NO: 1.