发明名称 |
CARRIER-LIGAND FUSIONS AND USES THEREOF |
摘要 |
Improved methods for purification, detection or characterization of molecules have been described in embodiments of the present invention. The improved methods rely on the ability to create a carrier reagent which is capable of (i) binding to a matrix either specifically or non-specifically; and (ii) forming a covalent linkage with any ligand having a nucleophilic group or a thioester as a result of a simple reaction which does not require a variety of chemical reagents or sophisticated chemistry. The covalent linkage between the carrier and the ligand relies on the chemical reaction between a thioester and a nucleophilic group. The carrier should either contain a reactive thioester which if it is a protein should be at the C-terminal end of the protein or a reactive nucleophilic group which if it is a protein should be at the N-terminal end and should preferably be a cysteine or selenocysteine. The ligand requires a reactive nucleophilic group to react with the thioester on the carrier or if the carrier has a reactive nucleophilic group, the ligand should have a reactive thioester. |
申请公布号 |
WO2004053460(A2) |
申请公布日期 |
2004.06.24 |
申请号 |
WO2003US39350 |
申请日期 |
2003.12.11 |
申请人 |
NEW ENGLAND BIOLABS INC |
发明人 |
XU MING-QUN;SUN LUO;GHOSH INCA;EVANS THOMAS C |
分类号 |
C07K16/18;C12N9/00;G01N;G01N33/532;G01N33/543 |
主分类号 |
C07K16/18 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|