Identification of protein interactions using in vivo post-translationally modified fusion proteins

摘要

Methods and products for the study of in vivo protein interactions and the identification of natural ligands or binding partners for proteins are disclosed. The methods employ fusion proteins comprising a protein of interest and a post-translational modification sequence which is post-translationally modified with a tag. The tag can then be used in an affinity purification methodology to extract both the fusion protein and its natural ligands or binding partners from a cell lysate. Also disclosed are vectors useful in producing such fusions, and cells transformed with such vectors.