CELL EXTRACT OF ESCHERICHIA COLI HAVING MUTATION IN S12 RIBOSOMAL PROTEIN AND PROCESS FOR PRODUCING PROTEIN IN CELL-FREE SYSTEM USING THE SAME

摘要

It is intended to provide an extract, a kit and a method for synthesizing a protein in a cell-free system. The above-described extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in an S12 ribosomal protein gene. In a preferred embodiment, the above mutation is such a mutation as imparting tolerance to streptomycin or dependence thereon to E. coli whereby the reading efficiency of mRNA codon on ribosome can be improved and thus the protein productivity can be elevated.