| 摘要 |
PURPOSE: PCR general primers and method for detecting various type of human papillomavirus(HPV) by PCR are provided, thereby diagnosing cervical cancer at an early stage. CONSTITUTION: The PCR general primers for detecting various type of human papillomavirus(HPV) comprises a first primer having the nucleotide sequences set forth in SEQ ID NO: 1 and SEQ ID NO: 2, a second primer having the nucleotide sequences set forth in SEQ ID NO: 3 and SEQ ID NO: 4, and a third primer having the nucleotide sequences set forth in SEQ ID NO: 5 and SEQ ID NO: 6, wherein the first primer amplifies HPV types 1a, 2a, 3, 4, 6b, 7, 10, 11, 13, 16, 16r, 18, 26, 27, 28, 30, 31, 32, 33, 34, 35, 35h, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 65, 66, 67, 68, 70, 72, 73, 74, and 77 DNA; the second primer amplifies HPV types 1a, 2a, 3, 4, 6b, 7, 10, 11, 13, 16, 16r 18, 26, 27, 28, 30, 31, 32, 33, 34, 35, 35h, 39, 40, 42, 43, 44, 45, 48, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 65, 66, 67, 68, 70, 72, 73, 74 and 77 DNA; and the third primer amplifies HPV types 2a, 3, 6b, 10, 11, 13, 16, 16r, 18, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 35h, 39, 40, 42, 43, 44, 45, 48, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 63, 66, 67, 68, 70, 72, 73, 75, 76 and 77 DNA.
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