METHOD FOR GENERATING IMMUNOGLOBULIN GENES

摘要

The invention provides a method for preparing a double stranded nucleic acid which encodes an immunoglobulin, comprising the steps of: (a) providing a set of three or more overlapping oligonucleotides which anneal to form the + and - strands of a nucleic acid which encodes at least part of an immunoglobulin variable domain; (b) annealing the oligonucleotides; (c) replicating the + and - strands of the nucleic acid formed from the annealed oligonucleotides; and (d) inserting the nucleic acid into an expression vector.