发明名称 |
Rapid fluorescence detection of binding to nucleic acid drug targets labelled with highly fluorescent nucleotide base analogs |
摘要 |
The present invention is a method of identifying ligands that interact will cellular processes involved in the life-cycle of HIV. In particular, an oligonucleotide, corresponding to specific RNA sequence within an infected cell, is modified by the substitution of 2-aminopurine. As a result, interactions between the oligonucleotide and the ligand can be measured via fluorescence. This technique can be use to find inhibitors of binding between rev and its response element (RRE), dimerization initiation sequences, and topoisomerases and DNA.
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申请公布号 |
US6743586(B2) |
申请公布日期 |
2004.06.01 |
申请号 |
US20010983742 |
申请日期 |
2001.10.25 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE COMMERCE |
发明人 |
MARINO JOHN P.;STIVERS JAMES T.;LACOURCIERE KAREN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;G01N33/53;G01N33/566;C07K14/00;C07H21/02;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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