| 摘要 |
The present invention provides nucleic acid molecules encoding a fluorescent and chromo- proteins and mutants, variants and derivatives thereof, as well as proteins and peptides encoded by these nucleic acids. The nucleic acid molecules and proteins of interest are isolated from non-Aequorea Hydrozoa species. The proteins of interest include yellow fluorescent protein, phiYFP , from Phialidium sp., green fluorescent protein hydr1GFP and purple chromoprotein, hm2CP from hydroid medusae of sub-order Anthomedusae. Also of interest are proteins that are substantially similar to, or derivatives, or homologues, or mutants of, the above-referenced specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby , as well as antibodies specific to the proteins and peptides of the invention . In addition, host-cells, stable cell lines and transgenic organisms comprisi ng above-referenced nucleic acid molecules are provided. The subject protein an d nucleic acid compositions find use in a variety of different applications an d methods, particularly for labeling of biomolecules, cell or cell organelles. Finally, kits for use in such methods and applications are provided.
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